Expresión circadiana específica de la localización de leptina y su receptor en tejido adiposo humano
2011
Introduction: Circadian variability of circulating leptin
levels has been well established over the last decade.
However, the circadian behavior of leptin in human adipose
tissue remains unknown. This also applies to the soluble
leptin receptor.
Objective: We investigated the ex vivo circadian behavior
of leptin and its receptor expression in human adipose
tissue (AT).
Subjects and methods: Visceral and subcutaneous
abdominal AT biopsies (n = 6) were obtained from morbid
obese women (BMI 40 kg/m2). Anthropometric variables
and fasting plasma glucose, leptin, lipids and lipoprotein
concentrations were determined. In order to investigate
rhythmic expression pattern of leptin and its receptor, AT
explants were cultured during 24-h and gene expression
was analyzed at the following times: 08:00, 14:00, 20:00,
02:00 h, using quantitative real-time PCR.
Results: Leptin expression showed an oscillatory pattern
that was consistent with circadian rhythm in cultured
AT. Similar patterns were noted for the leptin
receptor. Leptin showed its achrophase (maximum
expression) during the night, which might be associated
to a lower degree of fat accumulation and higher mobilization.
When comparing both fat depots, visceral AT
anticipated its expression towards afternoon and evening
hours. Interestingly, leptin plasma values were associated
with decreased amplitude of LEP rhythm. This association
was lost when adjusting for waist circumference.
Conclusion: Circadian rhythmicity has been demonstrated
in leptin and its receptor in human AT cultures in
a site-specific manner. This new knowledge paves the way
for a better understanding of the autocrine/paracrine role
of leptin in human AT.
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