Primers, kit and detection method for detecting gene type of dominant white feather site of chicken PMEL17 gene

The present invention discloses primers, kits and detection methods for detecting chicken PMEL17 dominant gene feather locus genotypes, the field of biotechnology. The present invention is directed to the first exon 10 PMEL17 gene 9bp insert (-CTGGGCACC-) designed allele-I specific primers P1-R; for exon 10 PMEL17 exon no 9bp insert design allele-i-specific primer P2- F.; the I allele-specific primer pairs P1 amplified chicken PMEL17 gene I allele to i allele-specific primer pair P2 amplified chicken PMEL17 gene i allele, subjected to agarose gel after the PCR reaction electrophoresis, according to agarose gel electrophoresis and accurate, rapid and convenient detection PMEL17 dominant gene feather locus genotypes. Compared with the prior art, molecular biology methods of the invention established greatly improved genotypes determined efficiency and accuracy, the method is simple, easy to operate, and low cost.