Evolution of circulating tumor cells (CTCs) during neoadjuvant treatment and radical surgery in patients (pts) with liver-only metastases of colorectal cancer (L-CRC).

e14146 Background: The presence of baseline ≥ 3 (CTCs) in 7.5 mL of blood is predictive of a poor outcome in advanced colorectal cancer (JCO, 26;19: 3213-3221, 2008). The aim of this prospectively. study was to quantified CTCs performance during neoadjuvant treatment and before/after radical surgery in pts with L-CRC. Methods: From February 2009 to October 2010, 18 pts with L-CRC were included. Median age was 61 years old (48-79), 55.6% men. K-Ras status was: 54% wild-type; 46% mutated. 7.5 mL of blood were drawn in CellSave tubes. CTCs were isolated and enumerated at baseline, before and just performed radical liver surgery. CTCs were immunomagnetically separated and fluorescently labeled using the CellSearch System (Veridex/Immunicon Corp.). Results: Baseline determination was < 3 CTCs in 72.2 % of pts, and increased to 77.8% just before liver surgery. A slight decreased in pts with < 3 CTCs was observed after liver surgery (70.6%). Conclusions: Unlike the published data of CTCs before/after liver surge...