Comparison between Maize Mature Embryo and Shoot Tip Callus Induced and Plant Regeneration

This paper studies the initiation,maintenance and differentiation of callus from mature embryo and shoot tip of three genotypes in corn.The results showed that maize mature embryo and the callus induced frequency were correlated between mature embryo and shoot tip,but the callus quality was obviously different.Callus obtained from shoot tip were generally better than that of mature embryo.The differentiation frequency had great difference between these two kinds of callus, only 2.13% differentiation frequency could be obtained on mature embryo callus,and the differentiation frequency on shoot tip callus could be up to(21.2%).