Packing method and detecting method of gel chromatography column

The present invention provides a gel chromatography column packing method, comprising the steps of: 1) preparing the gel suspension: Add 0.02-0.4mol / L NaOH solution containing NaCl and a phosphate buffer solution in the gel, prepared mixed gel suspension base salts; 2) packed: the bottom of the vial of purified water was added and the gel slurry into the column tube; pumped NaOH solution and phosphate buffer solution containing 1: 1.0-3 mixing alkaline salt buffer, when the gel suspension until the liquid level constant, a next equalization step begins; 3) balance: equilibrated with a buffer containing NaCl, 1-3 column volumes; 4) column efficiency testing: with NaCl as a tracer to monitor changes in conductance value, to obtain effective results column. Packed using the method of the present invention compared with the conventional column packing method, the resulting packed effect is the same, except that the new method can greatly improve the packed column packing efficiency, overcoming the repeated large volume of packed and balance defects.