Linkage analysis of the murine Hyal-1 locus on chromosome 9

We have recently described a new locus, Hyal-1, which determines hyaluronidase variants in mouse serum. On the basis of segregation in recombinant inbred and congeric strains, Hyal-1 was tentatively assigned to chromosome 9 (Fiszer-Szafarz and De Maeyer, '89). In the present study we have performed a linkage analysis of Hyal-1 using 156 backcross progeny of an interspecies cross of laboratory mice and Mus Spretus. Linkage was tested to two anchor loci on chromosome 9: d (dilute, a coat color locus) and Bgl-s (a locus controlling β-galactosidase activity). The gene order (from centromere) with intervening percentage recombination is d-16.6 ( ± 2.9)-Hyal-1-10.9 ( ± 2.4)-Bgl-s, indicating close linkage to H-7 and Fv-2.