A Helpful PEST for Listeriolysin O

Listeriolysin O (LLO) perforates host vacuoles, allowing Listeria monocytogenes to escape to the cytosol. How cytosolic LLO prevents cell lysis was not understood. In this issue of Cell Host & Microbe , Chen et al. (2018) show that a PEST sequence prevents cytotoxicity by mediating LLO endocytosis from the plasma membrane.