Screening Wound-Healing Potential of Different Aloe vera L. Germplasms at the Cellular Level

Aloe vera L. germplasms were tested for their ability to heal wounds following proliferation and migration of murine L929 fibroblast cells employing MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and an in vitro scratch assay. All the germplasms were capable of promoting cell proliferation and migration. A positive correlation was observed in proliferation and migration of cells among the aloe germplasms independent of treatment concentration. Of the tested germplasms, TN exhibited maximum while RJN showed minimumwound-healing potential. This ability of A. vera to stimulate fibroblast proliferation and migration might help skin regeneration and repair. The in vitro scratch assay employed in the present study for the first time documents its efficiency in evaluating wound-healing potential following fibroblast migration.