Plasma MiRNA alterations between NSCLC patients harboring Del19 and L858R EGFR mutations

// Yihan Ma 1 , Peiqi Xu 2 , Yanjun Mi 1 , Wenyi Wang 1 , Xiaoyan Pan 1 , Xiaoting Wu 1 , Qi He 1 , Hongming Liu 1 , Weiwei Tang 1 , Hanxiang An 1 1 Xiamen Cancer Hospital, The First Affiliated Hospital of Xiamen University, 361003, Fujian, China 2 Reproduction Center, The Second Affiliated Hospital of Kunming Medical University 650101, Yunnan, China Correspondence to: Hanxiang An, email: anhanxiang@xmu.edu.cn Keywords: circulating miRNA, microarray, NSCLC, EGFR, response Received: April 29, 2016      Accepted: July 10, 2016      Published: July 24, 2016 ABSTRACT Based on recognition of driver mutations, treatment paradigm for non-small-cell lung cancer (NSCLC) patients has been shifted. However, recently exon 19 deletion mutation (del19) of epidermal growth factor receptor (EGFR) clearly shows better clinical benefit over single-point substitution mutation L858R in exon 21 (L858R). The aim of this study was to investigate the difference by analyzing the expression of plasma microRNAs (miRNAs) of NSCLC patients with EGFR mutation del19 or L858R. MiRNA microarray of plasma from patients’ blood identified 79 mapped, network-eligible miRNAs (fold > 5), of which 76 were up regulated and 3 were down regulated. Genetic network was performed with Ingenuity Pathway Analysis (IPA). Among analysis, MYC, Argonaute2 (AGO2), Y-box binding protein 1 (YBX1), cyclin E1 (CCNE1) were involved in organismal abnormalities and cancer. Our findings provide information on the epigenetic signature of the two major sensitive mutations among NSCLC and add to the understanding of mechanisms underlying the different outcomes.