超顺磁性氧化铁标记人Flk-1(上标 +)CD31(上标 -)CD34(上标 -)间充质干细胞对细胞增殖及向神经细胞分化的影响

Objective To label Flk-1(superscript +)CD31-CD34-human mesenchymal stem cells (hMSCs) with superparamagnetic iron oxide (SPIO) and to evaluate the effect of SPIO on proliferation and neural differentiation of labeled cells. Methods hMSCs were incubated with SPIO (50 mg/L) and PLL (1.5 mg/L) overnight (12~18 hours). Both labeled and unlabeled cells went through growth curve test, Trypan blue staining and flow cytometer to evaluate the effects of SPIO on cell proliferation, cell viability and surface markers. Immunofluorescence assay was conducted for neuron and neuroglia specific cell surface markers after neural induction protocols were used. Results Cell viability of the two groups were both more than 90% for 7 days. There was no significant difference in cell viability and growth curve test between two groups. The results of flow cytometer showed that both labeled and unlabeled cells expressed CD44, CD105 and Flk-1 markers, while CD31 and CD34 were negative. After neural induction, the statistical analysis of A value for all the markers showed no significant difference between the two groups. Conclusion SPIO, as MRI cellular contrast, is safe and efficient.