Key Parameter Affecting the Quality of High-Throughput DNA Sequencing in Rice

By using rice plasmid as materials, the feature of MegaBACE 1000 DNA analysis system and the key parameters affecting the quality of DNA sequencing were analyzed. It showed that the quality of plasmid DNA was the key parameter affecting sequencing, and the homogeneity of the sequencing reaction products in the same plate was critical to improve efficiency of high-throughput DNA sequencing.