Abstract 15292: Novel Approach to Prevent Systemic Inflammation Following Prolonged Cardiopulmonary Bypass Using Allogeneic Administration of Fetal Membrane-Derived Mesenchymal Stem Cells in Rats

Background Systemic inflammation after prolonged cardiopulmonary bypass (CPB) often causes serious multi-organ system dysfunction. Mesenchymal stem cells (MSCs) are reported to reduce inflammation and attenuate cell-mediated immune response. We have focused on fetal membranes (FMs) as an alternative source of MSC to provide a large number of cells and previously reported allogeneic administration of FM-MSCs attenuate autoimmune myocarditis (JMCC 2010), and glomerulonephritis (AJP 2010). The aim of this study was to investigate whether allogeneic FM-MSCs attenuate systemic inflammatory response in a rat CPB model. Methods and Results Male Lewis rats were randomly divided into two groups (n=7 each): CPB alone (control group) and CPB+MSCs (MSC group). Rat CPB models were established by arterial infusion through the left femoral artery and venous drainage through the right jugular vein into the right atrium, and the flow rate was adjusted to 50ml/kg/min and maintained for 30 minutes. In the MSC group, MSCs (1...