Rational designed benzochalcone-based fluorescent probe for molecular imaging of hydrogen peroxide in live cells and tissues

Abstract Hydrogen peroxide (H 2 O 2 ), a member of reactive oxygen species (ROS), usually acts as a significant oxidative stress marker in some diseases for its abnormal expression. Due to the merits of non-invasive and high selectivity, fluorescent probe has been proved to be an efficient method for H 2 O 2 detection. Herein, we presented two novel boronate probes FD-1 and FD-2, in which the C-B bonds are well suitable for high selective detection of H 2 O 2 . Notably, these two probes were based on 4-hydroxybenzochalcone fluorophore, which exhibits excellent fluorescent properties through the intramolecular hydrogen bond. The results demonstrated that these two probes displayed satisfactory sensitivity and selectivity to H 2 O 2 , and probe FD-2 displayed faster response rate to H 2 O 2 than FD-1. Importantly, FD-2 was successfully applied to image H 2 O 2 in living cells and tissues, indicating probe FD-2 has great potential to monitor the H 2 O 2 in biological system.