Magnetic particle chemiluminescence kit for detecting nitrofurantion and application thereof

2010 
The invention relates to a magnetic particle chemiluminescence kit for detecting nitrofurantion. The kit comprises the following reagents: a luminescent label, a fluorescent label, a standard product, a quality control product and a separating reagent. The luminescent label is of a nitrofurantion hapten labeled by an isoluminol luminescent label, the fluorescent label is of a nitrofurantion monoclonal antibody labeled by fluorescein or derivatives thereof, and the separating reagent is of paramagnetic nano micro-beads coated with a goat anti-FITC (fluorescein isothiocyanate) monoclonal antibody. The invention further relates to a method for detecting the nitrofurantion in feed by adopting the kit, and the method has the advantages of higher sensitivity in detection of the nitrofurantion, higher specificity and faster detection speed.
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