Effects of hypoxic cell radiosensitizers on the activity of ATPases

Misonidazole (MIS, Ro-07-0582, 1-(2'-hydroxy-3'-methoxypropyl)-2nitroimidazole) was a gift from Hoffman-La Roche Ltd. Basle, Switzerland ; metronidazole (MET, 1-(2'-hydroxyethyl)-2-methyl-5-nitroimidazole) was purchased from May and Baker Ltd . England; PNAP (p-nitroacetophenone) and NDPP (Ro-03-6156, p-nitro-(3-dimethylamino)-propiophenone) were a gift from Dr . L. Revesz of the Karolinska Institute, Stockholm ; DNP (2,4-dinitrophenol) was provided by Fluka A .G.,Switzerland; ATP (adenosine-5'-triphosphoric acid disodium salt) was from Merck, Germany ; Tris (Trishydroxymethylaminomethane) was from Loba-Chimie, Wien ; EDTA (ethylendiaminotetraacetic acid) and dextran sulphate 500 were obtained from Sigma Chemical Co