Active Ingredient Estimation of Clopyralid Formulation by Reversed Phase HPLC

A simple, accurate, and reliable high-performance liquid chromatography (HPLC) method was developed for determination, separation and estimation of clopyralid active ingredient in its formulation. The active ingredient in formulation was estimated by two methods and cleanup was performed using C18 SPE. Estimation was done by HPLC using 5 μm, C18 column, and mobile phase 0.02 % acetic acid in methanol: acetonitrile (90:10 v/v) and detection at 229 nm. The efficiency of clean up for standard clopyralid was found to be 98.02-98.66% at different concentrations. Overall recoveries for different methods ranged from 98.3-100.1 %. The LOD and LOQ were 0.1 and 0.3 ng respectively. The detector response was linear within concentrations range 0.25-10.0 ng at RSD 1.87%.