Diversidade genética de Cucurbita pepo, C. argyrosperma e C. ficifolia empregando marcadores microssatélites
2013
Genetic diversity of Cucurbita pepo, C. argyrosperma and C. ficifolia accessions evaluated through microsatellite markers Landraces of five domesticated species of Cucurbita are cultivated in southern Brazil. Among them C. argyrosperma and C. ficifolia are the least studied. Microsatellite markers are important for the molecular characterization of these two species, but there is a need for specific primers, not yet available. The aim of this study was to evaluate the transferability of primers and optimize the amplification of SSR loci in C. pepo, C. argyrosperma and C. ficifolia and also evaluate genetic diversity in different accessions of germplasm in these species. We used ten accessions of C. pepo, nine of C. argyrosperma and five of C. ficifolia from the Genebank of Cucurbitaceae from Embrapa Temperate Agriculture. DNA was extracted in bulk from the leaves of five individuals of each accession. 40 SSR primers developed for C. pepo were used. In C. pepo 35 primers (87.5%) amplified, in C. argyrosperma, 34 primers (85%), and in C. ficifolia , 32 primers (80%). The transferability of SSR loci in C. pepo to C. argyrosperma and C. ficifolia was 85 and 80% respectively. The transferability of SSR loci from C. pepo to C. argyrosperma and C. ficifolia was 85 and 80% respectively. Thus, the transferability rate of SSR loci developed for C. pepo to C. argyrosperma and C. ficifolia was high, indicating that they can be used in molecular characterization of these species.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
20
References
2
Citations
NaN
KQI