血红素加氧酶－1上调CD4(上标 +)CD25(上标 +)Treg foxp3表达以增强Treg的免疫抑制功能

To explore the expression of CD4(superscript +) CD25(superscript +) regulatory T cells (Treg) foxp3 and the increase of IL-10 secretion induced by heme oxygenase-1 (HO-1) to enhance Treg immunosuppressive function. CD4(superscript +) 25(superscript +) Treg from BALB/c mouse spleen were negatively isolated by microbeads, and the cells were transfected by plasmids pcDNA3 and pcDNA3HO-1 or treated with hemin and Sn-protoporphyrin (SnPP), respectively. BALB/c mouse was sensitized and challenged by ovalbumin (OVA) and administrated with hemin or SnPP. The expression levels of HO-1, foxp3 mRNA and the proteins production were analyzed in CD4(superscript +) CD25(superscript +) Treg, and concentration of IL-10 as well as TGF-β in culture supernatants and serum were measured. Then the suppressive capacity of CD4(superscript +) CD25(superscript +) Treg was detected. The results showed that the expression levels of foxp3 mRNA and the protein production were enhanced after CD4(superscript +) CD25(superscript +) Treg transfected by pcDNA3HO-1 and treatment with hemin, the concentration of IL-10 was increased in culture supernatant and in serum of BALB/c mouse sensitized and challenged by OVA and hemin. The suppressive capacity of CD4(superscript +) CD25(superscript +) Treg was also increased in group of mice treated with hemin. This study indicates that HO-1 induced the upregulation of CD4(superscript +) CD25(superscript +) Treg foxp3 mRNA and the protein production, promote the excretion of IL-10, and enhance immunosuppressive function.