The effect of semen extender and storage time on the quality of spermatozoa collected from the excurrent duct of Philippine local chicken

The study was conducted primarily to determine the effect of AU and Lake's Low Temperature (LLT) extenders and to determine the effect of extended semen at 0 hour, 24 hours, 48 hours and 72 hours storage times. Twelve roosters were used for the collection of spermatozoa from the excurrent duct (epididymis and vas deferens) of Philippine local chickens. During the time of semen storage, a decrease in the number of motile, live, morphologically normal spermatozoa and an increase in dead and abnormal spermatozoa were observed. The LLT extender was found to be a more suitable extender because the number of live spermatozoa did not decrease as much as in semen diluted with the AU extenders. In addition, the best time storage determined was between 0 hour to 24 hours on both extenders, however, results showed that microscopic evaluation at 24 hours to 48 hours in LLT extender had comparable semen quality.