An Efficient And Quick Protocol For In Vitro Multiplication Of Snake Plant, Sansevieria Trifasciata Var. Laurentii [Prain]

An efficient protocol was developed for quick propagation of snake plant under in vitro conditions. Leaf segments sized 1 cm were surface sterilized and inoculated on Murashige and Skoog media supplemented with 3% sucrose, 0.8% agar, and various concentrations of indole-3-butyric acid (1 to 10 mg/L). Cultures were maintained for 4 to 5 weeks at standard conditions to allow root induction and elongation. Shoot induction was triggered upon altering the daytime culture room temperature to 37 ± 2 °C. Multiple shoots were produced at higher IBA concentrations. Another 5 weeks later, individual plantlets were excised and hardened for 2 weeks in soil preparation contained in small cups before transferring to 30 × 20 × 20 cm sized pots. We discuss the probable events effectuating unusual shoot regeneration at relatively higher temperatures in media without supplementing any cytokinins. This manuscript offers a unique protocol for rapid in vitro regeneration in snake plants using leaf explants cultured in airtight-sealed vessels. The success of the protocol relies on IBA led root induction at standard tissue culture temperatures, while shoot induction uses only an abrupt daytime shift to relatively high temperatures.