Study on the antioxidant activity of flavonoids extracted from Lespedeza davurica

[Objective] The study discussed the antioxidant activity of Lespedeza davurica flavonoid(LDF)in vitro.[Method] Fenton reaction,pyrogallic autoxidation and I2-Na2S2O3 titration were used to determine its scavenging effect on hydroxyl radicals (·OH ) and superoxide anion(O2-) and inhibition on lard,colleseed oil and linoleic acid.[Result] LDF had good scavenging effect on hydroxyl radicals (·OH) and superoxide anion(O2-),in a certain scope,the effect grew stronger as mass concentration increased.When the mass concentration of additional LDF reached 1.0 mg/mL,the maximum scavenging rate on hydroxyl radicals (·OH) was 97.44%;when the mass concentration was 0.48 mg/mL,the maximum scavenging rate on superoxide anion(O2-) was 97.89%;LDF also had good antioxidation on lard,colleseed oil and linoleic acid.LDF could inhibit POV of lard and collessed oil and inhibit oxidation of linoleic acid significantly (P0.01),and the effect became stronger as mass concentration of LDF increased.[Conclusion] The LDF has significant antioxidant activity,and more attention should be paid to its drug value.