Determination of 3 Chloramphenicols Medicine Residues in Aquatic Products by UPLC-MS/MS

A method was developed for the simultaneous determination of chloramphenicol,thiamphenicol and florfenicol residues in aquatic products using ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry(UPLC-ESI-MS/MS).The homogenized samples were extracted with ethylacetate and the extracts were partitioned with n-hexane to remove lipids.Further chromatographic separation was carried out on UPLC C18 column.Multiple reaction monitoring(MRM) with electrespray ioniza tion in positive mode was used for detection.Quantitation limit was 0.10μg/kg for chloramphenicol,with goodlinearity ranged from 0.10-50.0μg/L.Average recoveries spiked in negative samples at the levels ofchloramphenicol from 0.10-2.00μg/kg were 84.7%-104.9%.Quantitation limit was 0.50μg/kg for thiamphenicoland florfenicol,and linearity rang was 0.50-50.0μg/L.Spiked at the levels from 0.50-10.0μg/kg,the averagerecoveries were 88.2%-104.1%for thiamphenicol and 91.9%-104.1%for florfenicol.The relative standarddeviations(RSD)for all intra-tests were less than 15%.With the advantages of simplicity,rapidity,highaccuracy and precision,this method could be suitable for the rapid detection of chloramphenicol drugs residuein aquatic products.