Investigation of the Tumorigenicity of Tumor Suppressor Gene Tip30 Modified ACC-M Cells in BALB/C Mice

Objective: To observe the changes of the tumor suppressor gene Tip30 on the growth of transplant tumor on BALB/C mice with high metastasis cells of human salivary adenoid cystic carcinoma. Methods: 30 BALB/C nude mice(four-week-old, weighted 18-20g), divided randomly into 3 groups with 10 in each group, were injected at submandibular with AdTip30/ACC-M , wt/ACC-M and AdLacZ/ ACC-M . Observed the tumor growth and survival period. Results: The results of AdTip30/ACC-M showed that the tumor occurs 3 days later than that of wt/ACC-M and AdLacZ/ACC-M, and the tumor grew much slower(P0.01); the survival period of AdTip30/ACC-M was (53.0±3.3)d , with two mice survives for good, while wt/ACC-M was (26±2.2)d and AdLacZ/ACC-M was (29±2.4)d. Conclusion: Tumor suppressor gene Tip30 could significantly inhibit the growth of human SACC cells in nude mice and prolong survival time.