Method Validation with Enzyme-linked Immunosorbent Assay for Clenbuterol Residue Detection

Hapten clenbuterol was conjugated with Bovine Sera Albumin (BSA) and Ovalbumin(OVA) by diazotization to produce immunogen BSA-CL and OVA-CL used for immunizing rabbit. The rabbit antiserum was purified with ammonium sulfate fractionation to prepare polyclonal antibody against clenbuterol. An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect and quantitate clenbuterol. The sensitivity of the assay is 1.452μg/L, and the standard curve is linear from 7.26 to 90.75μg/L.