Micropropagation of Cochlospermum religiosum (L.) Alston through nodal explant.

Cochlospermum religiosum (L) Alston is an important medicinal plant belonging to the family Cochlospermaceae. The gum, stem bark and root are traditionally used to cure different diseases. The present study aimed to develop the multiple shoots from Cochlospermum religiosum through nodal explants. Shoot induction was achieved on Murashiage and Skoog (MS) medium fortified with 2 mg1 BAP, 2 mg1 KN and 2 mg1 BAP with 0.1 mg1 IAA. The shoots were sub cultured after two weeks on different concentration of BAP for multiple shoot induction. After four weeks the shoots were excised and sub cultured for rooting on half strength MS medium fortified with BAP 0.5 mg1 and IAA 2.0 mg1. The in vitro generated plantlets were successfully acclimatized in pots containing vermiculite and soil. The survival rate is 50 to 65 percent.