Cell membrane stabilizing effect of ethanol and aqueous extract of Humbildtia vahliana wight an invitro study

Aim: The aim of present study was to determine the membrane stabilizing effect of ethanol and aqueous extracts of Humboldtia vahliana Wight on human red blood cell (RBC) membrane. Methods: It was an invitro study, where blood sample was collected from the human volunteers and specific concentrations were made by diluting with buffers. The cell suspensions were exposed to 100 to 1000 μg/mL solution of ethanol and aqueous extracts of Humboldtia vahliana. Cell membrane stability was analyzed with different conditions like change in temperature and pH. Diclofenac sodium was used as standard drug. Each concentration of extracts was performed in a triplicate manner and the data were calculated and presented as Mean with standard deviation. Results: Membrane stabilizing activities were significantly higher with cells which were exposed to ethanol extract of Humboldtia vahliana than aqueous extract. The inhibition of cell membrane rupture was concentration dependent. Conclusion: This study results indicated that both extracts has cell membrane stabilizing activity on human RBC but ethanol extract was potent than aqueous extract. Study report suggested that Humboldtia vahliana bark extracts may offer some beneficial effects in the management of inflammatory mediators.