Glycoconjugate Vaccines Used for Prevention from Biological Agents: Tandem Mass Spectrometric Analysis
2014
In this review, we present the determination of the various glycation sites of synthetic neoglycoconjugates formed by conjugation of the antigenic saccharide hapten to BSA using tandem mass spectrometry. The ratio of hapten: BSA was determined by the matrix-assisted laser desorption/ionization-TOF/TOF-MS analyses of the glycoconjugates. We also tentatively propose that all glycated residues are located mainly near the outer surface of the protein.
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