The variations of and clinical significance of interleukin-5 and Interleukin-17 levels in children with different subtype asthma

Aim To investigate the effect of IL-5,IL-17 on the pathogenesis of moderate to severe children asthma in different subtypes.Methods ELISA was used to determine levels of IL-5 and IL-17 in peripheral blood mononuclear cells(PBMC)from 45 children with moderate to severe asthma in different subtypes and 20 healthy children as controls.PBMC were stimulated by anti-CD3 monoclonal antibody(OKT3)(0.2mg/L) and anti-CD28 monoclonal antibody(anti-CD28)(1mg/L).The relationship between IL-5,IL-17 and the disease severity and airway inflammation were also observed.Results The IL-5 level of EA group(79.75±13.49)pg/ml was significantly higher than that of NEA group(69.73±9.17) pg/ml and control group(51.30±14.33) pg/ml(P0.01).The IL-17 level of NEA group(5684.1±884.74)pg/ml was significantly higher than that of EA group(4938.1±465.58)pg/ml and control group(2685.0±1081.03)pg/ml(P0.01).The IL-5 and IL-17 level in chronic persistent group were significantly higher than those of clinical remission group(P0.05).Conclusion The IL-5 and IL-17 levels in PBMC of moderate to severe asthmatic children rised,and were related to the disease severtiy.