연구논문 : 명아주 에탄올 추출물의 프로콜라젠 I형 합성 촉진과 MMP-2 mRNA 발현 억제 효과

Chenopodium album (C. album) is well-known for a medical plant and nutritions. The aim of this study is to examine the anti-wrinkles activity from C. album as a functional cosmetic material. Cell viability as the safety was measured in keratinocytes and fibroblast by MTT-assay. Production of procollagen type I and expression of Col Iαl mRNA, MMP-1, 2 mRNA were observed for anti-wrinkles effect. When observing stability and the effect of improving wrinkles with C. album as a functional cosmetic component, cell survival rate was turned out to be less reduced in the C. album ethanol extract in the amount of 50 ppm. Therefore, cytotoxicity seems to be minor. The C. album ethanol extract had the expression of Collage Iα1 and synthesis rate of pro-collagen I-type that were slightly lower than the TNF-β1, a positive-contrast substance, in the Hs 683. However, they were significantly higher than the control (p<0.05). The expression of MMP-1 mRNA, a collagen breakdown enzyme, was lower than the Vit. C 250 nM, a positive-contrast substance, but was similar with the control. However, the expression of MMP-2 mRNA was higher than the control and Vit. C 250 uM in the 10 ppm. Therefore, C. album ethanol extract promotes the synthesis of pro-collagen I-type and the expression of Collage Ia1 mRNA in the in vitro experiment and also is outstanding in suppressing the expression of MMP-2 mRNA, a collagen and elastin breakdown enzyme. Therefore, C. album ethanol extract is highly usable as a new cosmetic material for improving wrinkle. Hence, it is recommended to conduct more in-depth research in regard of elastin as well as concentration, dosage form, and collagen.