Quantitative analysis of β-exotoxin in insecticidal preparations by high-performance anion-exchange chromatography

A quantitative method is proposed for determining β-exotoxin, which is a product of the vital activity of certain serotypes ofBac. thuringiensis. The method is based on the use of high-performance anion-exchange chromatography. The chromatography of β-exotoxin-containing preparations was performed on a column (1.6 × 150 mm) containing type 2632 anion-exchange resin (Hitachi) in a concentration gradient of HCl and NaCl. The time of analysis amounted to 34 min. The method permits the analysis of β-exotoxin in insecticidal preparations in an amount of 1 µg and upwards.