Determination of clenbuterol residual in pork by flow injection chemiluminescence immunoassay

A sensitive and rapid immunoassay based on flow injection chemiluminescence detection has been developed for the determination of clenbuterol residual in pork.The chemiluminescence reaction of luminal and hydrogen peroxide-Urea adduct catalyzed by horseradish peroxidase(HRP) can be enhanced by 4-Biphenylboronic acid.After competitive immunoreaction,the supernate enzyme labeled compound was detected by flow injection chemiluminescence.Under the optimal conditions,the linear range was from 0.05 to 9 μg/L(r=0.9959),and the detection limit was 0.02 μg/L.The proposed method has advantages on sensitivity,simplicity,reliablity as well as cheapness on instrument,and has been successfully applied to the determination of clenbuterol residual in pork with satisfactory results,providing a useful monitoring method for food safety.