Phenylacetate affects the expressions of c-myc and Caspase-3 in rat c6 glioma cells

Objective To study the effects of differentiation inducer phenylacetate(PA) on the expression of c-myc and Caspase-3 in rat C6 glioma cells and explore the mechanism of PA action.Methods C6 glioma cells were cultured in vitro.In experiment group,C6 cells were induced by PA at concentration of 5.0mmol/L for 72 hours.In control group,no PA was administrated.The expression of c-myc and Caspase-3 was detected by using an immunocytochemistry technique.The differernces of the imaging presentation in the two groups were analyzed.Results The expressions of c-myc in control group and experiment group were 0.214±0.013 and 0.153±0.007,respectively,while the expression of Caspase-3 were 0.149±0.004 and 0.206±0.033,respectively.Conclusion PA can down-regulate the expression of c-myc and enhance the expression of Caspase-3,which might consequently induce apoptosis in C6 glioma cells.