R460 – Relation Between SPA and Symptoms of Patients With AR and NP

ProblemThe aim of this study was to examine if allergic rhinitis and nasal polyposis are associated with the level of surfactant protein-A.MethodsSinus mucosal biopsies were performed in patients with allergic rhinitis (n= 15), nasal polyposis (n=21) and controls (n= 10). Immunolocalization of surfactant protein was performed with antibodies to SP-A using Streptavidin Peroxidase Conjugated Method and indirect immunofluorescence method. Blood serums were obtained from three subjects in each group for enzyme-linked immunosorbent assay (ELISA) analysis of surfactant protein-A.ResultsBy ELISA, AR (n =15) and NP (n = 21) showed significantly decreased levels of SP-A when compared with controls (n= 10), although these two groups were not statistically significant. Immunohistochemical investigation showed intense SP-A staining in the nasal epithelium of each groups, but weak staining in patients with AR and NP.ConclusionWe report for the first time the expression of SP-A in both diseased and normal nasal mucosa ...