Establishment of method detecting CD36 expression on human platelet and its application

The individual with the deficiency of CD36 antigen on platelet displayed the risk of anti-CD36 immune reaction induced by transfusion,which is one of the reasons for platelet transfusion refractoriness(PTR).This study was purposed to detect the expression level of CD36 antigen on platelet by flow cytometry among aphoresis platelet donors of Hangzhou area,and the frequency of CD36 deficiency was analyzed.Platelet-rich plasma(PRP) was separated from fresh anticoagulant whole blood by centrifugation,then the platelets were washed and adjusted to 1×106.The platelets were incubated with FITC-labeled CD36 and PE-labeled CD41 monoclonal antibodies,then the expression level of CD36 was detected by flow cytometry.The CD36 expression on monocytes for the samples of CD36-deficiency on the platelets was further analyzed.The results showed that 7 samples with CD36 antigen deficiency were found in 192 aphoresis platelet donors.The frequency of CD36 deficiency was 3.6% and all of them were typeⅡ deficiency.The significant difference of CD36 antigen expression was observed in the platelet donors of Hangzhou population,among them 59 individuals with low expressed CD36 antigen and 126 individuals with highly expressed CD36 antigen were found according to the geometric mean fluorescence intensity.It is concluded that the CD36 antigen deficient phenotype existed in the population,these data will provide the information for research of the CD36 antigen distribution and help to solve the platelet transfusion refractoriness.