Serum perioperative profile of cytokines in patients with squamous cell carcinoma of the larynx.

OBJECTIVE: Altered immune, inflammatory, and angiogenesis responses have been noticed in head and neck cancer, and many of these responses have been associated with a poor clinical outcome. The objective of this study was to evaluate several immune mediators in the sera of patients with squamous cell carcinoma (SCC) of the larynx undergoing curative surgery in connection with clinicopathologic factors. METHODS: Multiplex analysis of cytokines (interleukin [IL]-6, IL-8, IL-10, tumour necrosis factor α [TNF-α], interferon-γ [IFN-γ]), chemokines (monocyte chemoattractant protein 1 [MCP-1], macrophage inflammatory protein 1α [MIP-1α], and epithelial neutrophil-activating protein 78 [ENA-78]), and growth factors (vascular endothelial growth factor and basic fibroblast growth factor) in the serum of patients with laryngeal cancer and healthy controls was performed using xMap technology. RESULTS: Patients with SCC presented an altered cytokine profile compared to healthy controls, both preoperatively (higher levels of IL-8 and IL-10) and postoperatively (higher values for IL-6, IL-8, IL-10, and TNF-α). Heavy smoking was associated with significantly lower levels of ENA-78 and higher levels of IL-8. CONCLUSION: Differences noticed in patients' immune mediator profiles seem to be attributable to both disease and treatment. Further longitudinal studies are necessary to elucidate the involvement of immune mediators in disease progression and clinical evolution.