Use of an Orthogonal System, RP-LC–HILIC, for Evaluation of Purity

The reliability of evaluation of compound purity depends on the analytical method selected. A suitable analytical method, including the modes of separation and detection, can provide reliable information about compound purity. Effective separation is the key to achieving precise results. In this work we developed a novel method, based on an orthogonal system, RP-LC-hydrophilic interaction liquid chromatography, for evaluating the purity of compounds isolated by preparative reversed-phase liquid chromatography. Because of its good orthogonality, good separation and more accurate assessment of compound purity could be achieved.