Development and application of a species-specific anti-KLH ELISA in Beagle dogs

Limited nonclinical immunotoxicity data are available in the dog, although this is a major nonrodent species in regulatory safety studies. The aim of the present study was to measure primary anti-KLH IgM and IgG responses in Beagle dogs treated with the reference immunosuppressive drugs cyclosporine and cyclophosphamide, using a dog-specific sandwich ELISA assay jointly developed in-house by CiToxLAB France and Atlanbio.