Evaluation of gene expression level of CDC5L and MACC1 in poor prognosis and progression of osteosarcoma

Abstract Current evidences have indicated that osteosarcomais strongly associated with abnormal genetic and epigeneticchanges that lead to the abnormal expression of oncogenesor methylation of tumor suppressor genes. In the presentstudy, MACC1 and CDC5L mRNA levels in the patients withosteosarcoma were evaluated using quantitative real-timePCR. Our results demonstrated that CDC5L mRNA levelswere higher in tumor tissues than in adjacent normal tissues(2.713 ± 0.738 vs. 1.071 ± 0.629; P < 0.05). Moreover,MACC1 was upregulated in tumor bone tissues than in adjacentnormal tissues (3.221 ± 0. 624 vs. 1.427 ± 0.456;P < 0.05). Our result demonstrated that high expression ofCDC5L was significantly related to advanced TNM stage(P = 0.032). No significant difference was determined betweenCDC5L mRNA expression and other clinicopathologicalparameters including age, gender, tumor diameter,location, tumor grade, and histological type. In addition, overexpressionof MACC1 was strongly correlated with advancedTNMstage (P=0.027) and high tumor grade (P= 0.035). Ourfindings indicated that mRNA level of CDC5L is correlatedwith advanced TNM stage, and MACC1 may be involved inprogression of osteosarcoma.Keywords Osteosarcoma . Expression . PCR . CDC5L .MACC1