Amplification of PRDM16, BMP7, PPARα, COX II and PGC-1α Genes Tupaia belangeri

In the present study, partial cDNA sequence of PRDM16, BMP7, PPARα, COX II and PGC-1α genes in Tupaia belangeri were amplificated and analyzed. The obtained partial sequences of PRDM16, BMP7, PPARα, COX II and PGC-1α in T. belangeri were 184 bp, 84 bp, 358 bp, 212 bp and 271 bp, respectively. The coding protein of these sequences included 61 amino acids in PRDM16; 27 amino acids in BMP7; 119 amino acids in PPARα, 70 amino acids in COX II and 87 amino acids in PGC-1α. Comparison of the amino acids of PRDM16, BMP7, PPARα, COX II and PGC-1α with the amino acids in many mammals found that the homology of amino acids was higher. The PRDM16, BMP7, PPARα, COX II and PGC-1α genes were used to reconstruct phylogenetic trees which showed that T. belangeri had the closest relationship relative to primates.