Study on the effects of Shengxue-zengbai Decoction on the hematopoietic reconstitution in bone marrow transplanted mice and its molecular mechanism

Objective To investigate the effect of Shengxue-zengbai Decoction on the expression of ICAM-1 and SCF in bone marrow tissue and on the mechanism of hematopoietic reconstitution in bone marrow transplantation (BMT). Methods The experimental animals were randomly divided into S groups: normal group received no treatment, BMT control group, G-CSF group, Shengxue group, and Shengxue +G-CSF group. BMT mouse models were established. Shengxue group, Shengxue + G-CSF group were given Shengxuezengbai Decoction. On days 9, 14, 24 after BMT, mice were killed and peripheral blood cells, bone marrow nucleated cells were detected. Histological observation of bone was made and the ICAM-1 and SCF expression were assayed by immunohistochemistry. Results In Shengxue-zengbai Decoction group, the peripheral blood cell, bone marrow nucleated cell on the days 9, 14, 24 after BMT were higher than the other control groups. The expression of ICAM-1 and SCF than in the other control groups (P . Conclusion Shengxue-zengbai Decoction enhances ICAM-1 and SCF expression in bone marrow after syngenic bone marrow transplantation in mice, which may be related to the mechanisms of its accelerating hematopoietic reconstitution in allogenic bone marrow transplantation. Key words: Blood deficiency;  Bone marrow transplant;  Hemoatopoietic reconstruction