The expression of truncated YggG protein and preparation of its polyclonal antibody

AIM: To express truncated YggG protein(TYP) in Escherichia coli and to prepare anti-TYPE antibody. METHODS: Truncated yggg gen e was amplified by PCR from the plasmid containing full length yggg gene and was cloned into the expression vector pDH2. The expression of TYP was achieved by thermal induction. Antiserum was prepared by immunizing rabbit with TYP, T he titer and specificity of polyclonal antibody were detected by Western blot. RESULTS: Thin-layer scan analysis showed that TYP accounted fo r about 37.1% of total bacterial protein .The antiserum was about 1∶ 2 000 in titier and highly specific. Full-lergth rggG protein coul d also be recognized by the antiserum. CONCLUSION: The T YP was highly expressed, and specific anti-TYP antiserum was prepared success fully.