Apolipoprotein E polymorphisms in Japanese patients with polypoidal choroidal vasculopathy and exudative age-related macular degeneration

Purpose To study the genotypes, allelic frequencies, and polymorphisms of apolipoprotein E (Apo E) in unrelated Japanese patients with polypoidal choroidal vasculopathy (PCV) or exudative age-related macular degeneration (AMD) and control subjects without macular degeneration. Design Cross-sectional study. Methods Blood samples from 225 subjects older than 50 years were used. The 225 subjects included 58 patients with PCV, 85 with AMD, and 82 without macular degeneration. Coding exons of the Apo E gene were amplified by polymerase chain reaction, and the DNA sequences were determined by direct sequencing with an automated sequencer. Results Apo E e3/e3 was the most frequent genotype with a prevalence of 79.3% in PCV patients, 76.5% in AMD patients, and 67.1% in the control subjects. However, the differences in the percentages were not statistically significant among the three groups. The most frequently found allele in the three groups was e3. Patients with PCV and AMD were less likely to have e2 and e4 than the control subjects, but the differences were not statistically significant. Five minor Apo E single nucleotide polymorphisms, including e5 and e7, were found. Conclusion Japanese patients with PCV and AMD were less likely to have e2 and e4 polymorphisms, but the differences from the normals were not statistically significant for the Apo E genotypes and allelic frequencies.