Amperometric Sarcosine Biosensor Based on Hollow Magnetic Pt-Fe3O4@C Nanospheres

Abstract Sarcosine is a recently identified biomarker for prostate cancer. However, the rapid detection methods for sarcosine are relatively lack because of the low concentration and the presence of complicated interfering substances in serum or urine. In this manuscript, hollow nanospheres of Fe 3 O 4 was synthesized and used as carrier to disperse Pt (Pt) nanoparticles. In order to achieve excellent electron transfer ability, we use polyaniline to coat Pt-Fe 3 O 4 nanoparticles, and pyrolyze the polyaniline to carbon (C). Thus, hollow magnetic Pt-Fe 3 O 4 @C nanocomposites with good electron transfer ability are formed. The Pt-Fe 3 O 4 @C nanocomposites have high catalytic activity and stability. The nanocomposites were immobilized on glassy carbon electrode (GCE) to construct a nonenzyme hydrogen peroxide (H 2 O 2 ) sensor (Pt-Fe 3 O 4 @C/GCE). We further construct a sensitive sarcosine biosensor by immobilizing sarcosine oxidase (SOx) on the Pt-Fe 3 O 4 @C/GCE. The high catalytic activity and good biocompatibility of Pt-Fe 3 O 4 @C nanocomposites greatly retained the bioactivity of immobilized SOx, and the prepared sarcosine biosensor has good electrocatalytic performance towards sarcosine. It has a linear detection range between 0.5-60 μM with a limit of detection (LOD) of 0.43 μM (the signal to noise ratio is 3), and the sensitivity is 3.45 nA μM -1 (48.8 nA μM -1 cm -2 ), which has the potential to be used for rapid screening of prostate cancer.