Rejection of H-Y Disparate Skin Grafts by Monospecific CD4+ Th1 and Th2 Cells: No Requirement for CD8+ T Cells or B Cells

We wished to determine whether CD4 + T cells could reject a skin graft that was discordant for a single minor transplantation Ag in the absence of CD8 + T cells or Ab. Transgenic A1(M) mice were constructed that express the rearranged Vβ8.2 and Vα10 TCR genes from a T cell clone that is specific for the male Ag (H-Y) in the context of H2-E k . In addition, the RAG-1 −/− background was bred onto these mice to eliminate any endogenous TCR rearrangements. As expected, clonal deletion was found to be complete in the thymus of male A1(M)×RAG-1 −/− mice, while only CD4 + T cells were positively selected and found in the periphery of females. Female A1(M)×RAG-1 −/− mice were able to rapidly reject (in + T cells or surface Ig + B cells were present. An immunofluorescent analysis of spleen cells after grafting showed that the majority of T cells expressed activation markers (CD44, CD25, and intracytoplasmic IL-2) and a significant proportion were making IFN-γ and IL-4. Surprisingly, the transfer of either Th1 or Th2 CD4 + T cell lines from these mice into T cell-depleted recipients was sufficient to cause a specific rejection of male skin.