Method for measuring kinetic parameters of enzyme catalyzed reaction through isothermal titer thermal method

The invention discloses a method for measuring kinetic parameters of an enzyme catalyzed reaction through an isothermal titer thermal method, and belongs to the technical field of detection of the kinetic parameters of the enzyme catalyzed reaction. The method for measuring the kinetic parameters of the enzyme catalyzed reaction through the isothermal titer thermal method comprises the steps that a substrate is dropwise added to an enzyme containing solution, the thermal power of a substrate and enzyme reaction is detected every time the substrate is dropwise added, the interval time of dropwise adding the substrate is set, the changes of the thermal power at different substrate concentrations are calculated, and the Michaelis constant Km and maximum enzymatic reaction rate vmax of the enzymatic reaction are measured. According to the method, traditional measurement of the kinetic parameters of the enzyme catalyzed reaction is improved, the advantages of being fast, micro, sensitive and easy to operate are achieved, consumption of enzymes and the substrate in measurement of the kinetic parameters of the enzyme catalyzed reaction can be effectively reduced, the enzyme catalyzed reaction measuring process is simplified, and the accuracy of the result is improved.