Prevalence and Antimicrobial Susceptibility Pattern of Methicillin-resistant Staphylococcus aureus (MRSA) Isolates and Inducible Clindamycin resistance in Staphylococcus aureus: study at a Tertiary Care Hospital in KIMS, Bhubaneswar, India.

Aims: Methicillin resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in hospitals with hospital based outbreaks world-wide. The resistance to antimicrobial agents among Staphylococci is an increasing problem. The pres ent study was carried out to estimate the prevalence of MRSA isolates in clinical specimens and to investigate the sensitivity pattern of those isolates against various antibiotics used for treating hospitalized patients. Attempt was also done to find out the percentage of Staphylococcus aureus having inducible clindamycin resistance (iMLS B) in our geographic area using D -test. We tried to ascertain the relationship between the isolates of Methicillin -resistant Staphylococcus aureus (MRSA) and inducible clindamycin resistant Staphylococcus aureus.Place and Duration of the Study: An ongoing study (July 2013 - June 2014) in the Department of Microbiology, KIMS. Methodology: A total of 529 isolates of S. aureus were identified by standard laboratory procedures including catalase test, slide and tube coagulase tests, β- haemolysis on blood agar and growth on mannitol salt agar. Subsequently antibiotic sensitivity pattern of S. aureus were determined by Kirby Baeur’s disc diffusion method. Conclusion: Findings presented in this study indicated a high level of resistance to widely used therapeutic agents. An appropriate knowledge on the current antibiotic susceptibility pattern of MRSA is essential for appropriate therapeutic scenario. Total no. of samp les 7371, Staphylococcus aureus isolated 529 (7.17%), MRSA isolated-190 (36%). Out of 74 CL sensitive MSSA, 45 (23.6%) showed D test positive, indicating inducible resistance and 29 (15.2%) showed D test negative which are truly CL sensitive. O ut of 31 CL sensitive 9(2.6%) were D test positive indicating inducible CL resistance and 23(6.7%) were D test negative indicating true CL sensitivity.