Gelatin Stabilized Silver Nanoparticle Provides Higher Antimicrobial Efficiency as Against Chemically Synthesized Silver Nanoparticle

Silver nanoparticles (AgNPs) have become widely used nanomaterial due to its potential application in biomedical and pharmaceutical areas, as due to their antibacterial property. Even though it has antibacterial activity it also shows toxicity at higher concentration in humans and other organisms. Taking into consideration the above mentioned concern, we have performed a comparative antibacterial and hemolytic efficacy of gelatin stabilized/coated AgNPs (G-AgNPs) versus uncoated AgNPs. For G-AgNPs UV–Vis absorption shows λ max at 420–425 nm, FT-IR confirms that presence of amide group indicates AgNPs is stabilized/coated inside the gelatin and TEM analysis of G-AgNPs shows average particle size of 4.3 ± 1.3 nm that are mono dispersed in nature. AgNPs and G-AgNPs were demonstrated for their antibacterial activity using well diffusion method, Minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC) and antibiofilm assay against 4 clinical pathogens. In addition, we have performed a hemolytic assay on human RBC cells. The results show that biocompatible polymer (Gelatin) coated silver nanoparticle (G-AgNPs) exhibits excellent antibacterial activity as well as a minimal hemolytic effect than uncoated AgNPs. Based on this study, we suggest that G-AgNPs can be used as a promising nanomaterial in pharmaceutical and biomedical applications.