EXTRACTION PROCEDURES AND CELL WALL COMPOSITION, INCLUDING SOME RESULTS WITH CORYNEBACTERIA

ABSTRACT Cell wall preparations have been fractionated with hot formamide, lysozyme and lysozyme after pre-N- acetylation. Also trichloroacetic acid, cold or at 37° C, removes polysaccharides and teichoic acids. Dimethylhydrazine, or dilute alkali, also cause specific dissolution of some cell wall components. Isolation of purified specific polymers from the cell walls of bacteria grown in controlled conditions may provide valuable taxonomic information.