STUDY OF FIBRINOGEN ADSORPTION BY BIOMATERIALS USED IN ORTHOPEDIC SURGERY MEASURED USING THE QCM-D SYSTEM

Introduction and Objectives: In this study we attempted to analyze the biocompatibility of 3 different materials used in Orthopedic Surgery, Titanium, Tantalum and Hydroxyapatite, measuring their adsorption of fibrinogen by means of real time in vitro studies quantifying the mass of fibrinogen that becomes deposited on them. Materials and Methods: We assessed fibrinogen adsorption on these biomaterials by means of quantitative studies using the QCM-D system and qualitative studies reviewing fibrinogen adhesion, by atomic microscopy. For the statistical analysis of the results of this study we used SSP 14.0. Results: The results of the measurement of fibrinogen adsorption on the biomaterials used proved that when low concentrations of fibrinogen were used, 33 ng/ml, Ti and Ha adsorbed fibrinogen relatively rapidly, but Ta does not. However, when we used high concentrations of fibrinogen, 100 ng/ml, Ta adsorption increased markedly. When concentrations greater than 1000 ng/ml of fibrinogen were used, Ta was the biomaterial that showed most fibrinogen adsorption. Discussion and Conclusions: The adsorption of proteins from body fluids is the first biological response to the implantation of a biomaterial, and this protein adhesion will guide cell adhesion on the implanted biomaterial. In our in vitro study, carrying out measurements in real time, we saw that Ta was the material with greatest biocompatibility.