Catabolic Response Profiles, Catabolic Uniformity and Richness as Microbiological Indicators in a Soil of Pergamino, Buenos Aires Province, with and without Previous Application of Glyphosate

Incubation assay in trays was performed with two soils with different application histories of glyphosate: no application, and previous application. The soils used were Typic Argiudolls of Pergamino, province of Buenos Aires, and the treatments were: control (no application), and 20, 200 and 2000 mg of active ingredient per kg of soil. Sampling was performed at the beginning (T0) and 45 days after (T45). Catabolic response profiles (CRP), catabolic richness and catabolic uniformity were determined according to the methodology based on measuring the differences in respiration induced by substrate in a short time (4 hours). The substrates used in this study were 20, namely, two amines, 5 aminoacids, two carbohydrates, and 11 carboxylic acids. The objective of this work was to compare soils with different histories of application of glyphosate, measuring its effect on catabolic response profiles, catabolic uniformity and catabolic richness. In this study, no differences were observed between catabolic richness among the different sampling times and doses of glyphosate applied. Glyphosate application affected the structure of the soil microbial communities. At the end of the test, soils with all doses of previous herbicide application showed greater catabolic uniformity than soils without previous application.